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chromosome walking meaning in English

染色体步查,染色体步移
染色体巡查法
染色体移动

Examples

  1. The chromosome walking adapter consisting of 40nt and 44nt oligonucleotides was designed and synthesized
    结果显示, b8和b8f具有弱的一过性毒力,而b8b菌株无毒力。
  2. All these results show that tyrp1 expression is consistent with the melanin accumulation time . ( 4 ) started with aco1 ( aconitase i ) , we got positive clone of aco1 then obtained 16 clones by chromosome walking
    ( 4 )以离id最近的aco1 ( aconitase1 ,顺乌头酸酶)基因为起点,设计引物筛选含aco1的bac克隆,用染色体步行的方法得到16个bac克隆。
  3. And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed . the genomic dna of b8 was isolated , digested with bamh i , and ligated to the adapter . using the two pairs of the primers , two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully . lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f , the other is part of the 728 bp of f fragment . this result makes it possible to continue to carry out chromosome walking , to clone and sequence the whole genes of b fragment and f fragment , and to reveal the antagonistic molecular mechanism of b8
    试验研究设计并合成了由40和44个碱基的寡聚脱氧核苷酸组成的染色体爬行接头,在接头序列和测定的f片段近tn5的序列上,设计了2对染色体爬行用的pcr引物,从b8菌株中提取基因组dna , bamhi酶切,与染色体爬行接头连接,依次用2对引物进行pcr ,扩增出239bp产物,经克隆、测序,发现其中18bp为扩增的相应于f片段在b8f菌株tn5插入位点对面的序列,其余则为f片段728bp序列的一部分,为进一步进行染色体爬行,克隆和测定整个b和f基因,揭示阳菌株的拮抗分子机制提供了技术资料贮备。

Related Words

  1. chromosomes inversion
  2. chromosome rejoining
  3. chromosome manipulation
  4. unineme chromosome
  5. sat chromosome
  6. acrocentric chromosome
  7. nonbanded chromosome
  8. headed chromosome
  9. chromosome assortiment
  10. accessory chromosome
  11. chromosome translocation
  12. chromosome unstability syndrome
  13. chromosome x
  14. chromosome y
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