chromosome walking meaning in English
染色体步查，染色体步移
染色体巡查法
染色体移动
Examples
- The chromosome walking adapter consisting of 40nt and 44nt oligonucleotides was designed and synthesized
结果显示， b8和b8f具有弱的一过性毒力，而b8b菌株无毒力。 - All these results show that tyrp1 expression is consistent with the melanin accumulation time . ( 4 ) started with aco1 ( aconitase i ) , we got positive clone of aco1 then obtained 16 clones by chromosome walking
( 4 )以离id最近的aco1 ( aconitase1 ，顺乌头酸酶)基因为起点，设计引物筛选含aco1的bac克隆，用染色体步行的方法得到16个bac克隆。 - And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed . the genomic dna of b8 was isolated , digested with bamh i , and ligated to the adapter . using the two pairs of the primers , two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully . lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f , the other is part of the 728 bp of f fragment . this result makes it possible to continue to carry out chromosome walking , to clone and sequence the whole genes of b fragment and f fragment , and to reveal the antagonistic molecular mechanism of b8
试验研究设计并合成了由40和44个碱基的寡聚脱氧核苷酸组成的染色体爬行接头，在接头序列和测定的f片段近tn5的序列上，设计了2对染色体爬行用的pcr引物，从b8菌株中提取基因组dna ， bamhi酶切，与染色体爬行接头连接，依次用2对引物进行pcr ，扩增出239bp产物，经克隆、测序，发现其中18bp为扩增的相应于f片段在b8f菌株tn5插入位点对面的序列，其余则为f片段728bp序列的一部分，为进一步进行染色体爬行，克隆和测定整个b和f基因，揭示阳菌株的拮抗分子机制提供了技术资料贮备。